Growth stimulants in plant extracts for Leuconostoc citrovorum.

The effect of folic acid upon the urinary excretion of the growth factor required by Leuconostoc citrovorum.

In a previous study it was observed that Leuconostoc citrovorum 8081 failed to grow on a synthetic medium adequate for the growth of Leuconostoc mesenteroides P-60 and other common assay organisms (1). However, the addition to the medium of certain extracts from liver permitted excellent growth of L. citrovorum. Similarly, folic acid stimulated growth of the organism but the amounts necessary w...

Investigations on the biosynthesis of citrovorum factor by lactic acid bacteria.

Sauberlich and Baumann (1948) have reported that Leuconostoc citrovorum, strain 8081, failed to grow on a synthetic medium unless the medium was supplemented with an unidentified growth factor isolated from liver extracts, designated citrovorum factor. More recent reports in the literature (Sauberlich, 1949; Nichol and Welch, 1950a) point to the conversion of pteroylglutamic acid to citrovorum ...

Some factors influencing carbon dioxide production by Leuconostoc citrovorum.

This study was undertaken to determine whether Leuconostoc citrovorum plays a role in carbon dioxide production in milk. The ability of L. citrovorum strains to produce gas was studied by two methods. A qualitative method, in which an agar plug was forced up the neck of a volumetric flask, measured gas visually. This method demonstrated that 0.25% yeast extract, in a milk medium at 30 C, stimul...

Synthesis of citrovorum factor by Leuconostoc citrovorum; potentiation by ascorbic acid.

Further studies on the factor required by Leuconostoc citrovorum 8081.

Recently it was noted that Leuconostoc citrovorum 8081 failed to grow on a synthetic medium unless supplied with certain preparations from liver or other crude materials (1). Commercial preparations useful against pernicious anemia proved to be particularly active for this strain of L. citrovorum. The present report deals with preliminary attempts to concentrate the active principle and to diff...